Software

Model-building and refinement into cryoEM density

Our cryoEM model-building and refinement tools are included as part of the Rosetta software package.

Tutorials

The tutorial is intended to introduce users to several different ways Rosetta may be used to solve various structure determination tasks given 3-5Å cryoEM density data.  These tutorials are based on a presentation at the hybrid methods conference in Lake Tahoe, March 2015.

Update August 2018: Minor bugfixes & documentation updates
Update May 2018
: Fix a few errors with the RosettaES tutorial
Update May 2017: Update Rosetta XML formatting and default energy functions corresponding to latest release of Rosetta

The complete tutorial: [instructions (pdf)] [all files (tgz)]

Download individual parts:

  • Part 1: Introduction to Rosetta for cryoEM  [pdf][tgz]
  • Part 2: Fragment-based structure refinement [pdf][tgz]
  • Part 3: Model rebuilding using RosettaCM [pdf][tgz]
  • Part 4: De novo structure determination [pdf][tgz]
  • Part 5: Rebuilding long protein segments [pdf][tgz]

Supplemental Materials from Wang et al., eLife 2016 [1][2][3]


Slides

From a workshop at Karolinska Institutet, August 2018

  • Lecture 1 – Intro to Rosetta for cryoEM modelling [pdf]

From the Jülich “Hands-on” Workshop on Computational Biophysics, August 2016

  • Lecture 1 – Intro to Rosetta for cryoEM modelling [pdf]
  • Lecture 2 – Advanced Rosetta topics [pdf]